Ultrasensitive Rat Insulin ELISA(10-1251-01)

Kit format: 1 x 96 wells

Measurement range: 0.02 - 1.0 μg/L

• Very low detection limit - 0.02 µg/L
• High specificity - Insignificant cross-reactivity to C-peptide and proinsulin
• No interference from complex sample matrices

Mercodia Ultrasensitive Rat Insulin ELISA provides a method for the quantitative determination of low levels of insulin in rat samples.

Test characteristics

Samples: Serum, EDTA plasma and cell culture medium.
Sample volume: 25 µL
Assay range: 0.02 - 1.0 μg/L
Detection limit: ≤ 0.02 µg/L
Incubation: 2 h+ 30 min

Specificity

The ELISA kit measures rat insulin to 100%.

Mouse insulin 75 %
Rat proinsulin I 8 %
Rat proinsulin II 51 %
Mouse proinsulin I 31 %
Mouse proinsulin II 51 %
Mouse c-peptide I n.d.
Mouse c-peptide II n.d.
Rat c-peptide I n.d.
Rat c-peptide II n.d.
Human insulin 167 %
Human proinsulin 75 %
Human C-peptide n.d.
Insulin lispro (Humalog®) 167 %